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| 产品名称： | BAG-85D10 |
|---|---|
| 商品货号： | TS259695 |
| <strong>Cell line </strong>： | BAG-85D10 |
| DSMZ no. ： | TS259695 |
| Species ： | confirmed as mouse by species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | hybridoma (CD31) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgG1) CD31; the cell line was established in 1988 by fusion of PX63AG8.653 cells with spleen cells of a mouse immunized with human peripheral blood lymphocytes from a patient with high numbers of gamma-delta T cells |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells, growing singly and in small clumps |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement" for the first 1-2 weeks post-thawing) |
| Subculture ： | split saturated culture 1:2 every 3 days; seed out at 0.2 x 106 cells/ml; maintain at 0.2-0.8 x 106 cells/ml |
| Incubation ： | at 37°C with 10% CO2 |
| Doubling time ： | about 72 hrs |
| Harvest ： | maximal density at about 0.8 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgG1 monoclonal antibody |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |