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| 产品名称： | OTH-74D4 |
|---|---|
| 商品货号： | TS259698 |
| <strong>Cell line </strong>： | OTH-74D4 |
| DSMZ no. ： | TS259698 |
| Species ： | murine (mouse) (Mus musculus) |
| Cell type ： | hybridoma (CD45RA) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgG1) CD45RA; the cell line was established in 1985 by fusion of PX63AG8.653 cells with spleen cells of a mouse immunized with an EBV-transformed B cell line from a patient with MHC class II deficiency |
| Reference(s) ： | 15170 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, single cells growing in suspension and some slightly adherent |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement" for the first 1-2 weeks post-thawing) |
| Subculture ： | seed out at 0.3 x 106 cell/ml; maintain at 0.2-0.5 x 106 cells/ml; split saturated cultures 1:2 to 1:3 every 2 days; cells do not grow without HFCS, but it can be used at only 50% (1:100) of the recommended concentration |
| Incubation ： | at 37°C with 10% CO2 |
| Doubling time ： | about 40-50 hours |
| Harvest ： | maximal density of about 0.6-0.8 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgG1 monoclonal antibody |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |