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| 产品名称： | VIP-VIIIC8 |
|---|---|
| 商品货号： | TS259704 |
| <strong>Cell line </strong>： | VIP-VIIIC8 |
| DSMZ no. ： | TS259704 |
| Species ： | confirmed as mus musculus origin by COI DNA barcoding |
| Cell type ： | hybridoma (CD2) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgG2a) CD2; the cell line was established in 1987 by fusion of PX63AG8.653 cells with spleen cells of a mouse immunized with fresh peripheral blood lymphocytes from patients with expansions of gamma-delta Tcells |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells growing in suspension |
| Medium ： | 80% Dulbeccos MEM (4.5 g/L glucose) + 20% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement") |
| Subculture ： | split saturated cultures 1:2 to 1:3 every 3 days; seed out at 0.8 x 106 cell/ml, best in 12-well-plate with HFCS; maintain at 0.3-0.5 x 106 cells/ml; some cells attach to the surface of the culture flask, but can be detached by tapping the flask; for the first 2 weeks, HFCS should be used which thereafter can be reduced to 50% or 25% of the recommended concentration; cells grow very slowly in the complete absence of HFCS |
| Incubation ： | at 37°C with 10% CO2 |
| Doubling time ： | about 40-50 hours |
| Harvest ： | maximal density of about 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgG2a monoclonal antibody |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |