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| 产品名称： | CF-1D12 |
|---|---|
| 商品货号： | TS259706 |
| <strong>Cell line </strong>： | CF-1D12 |
| DSMZ no. ： | TS259706 |
| Species ： | origin confirmed as mus musculus by COI DNA Barcoding |
| Cell type ： | hybridoma (CD52) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgG3) CD52; the cell line was established in 1989 by fusion of PX63AG8.653 cells with spleen cells of a mouse immunized with IL-2-driven T cell lines from patients with high numbers of gamma-delta T cells in the peripheral blood |
| Reference(s) ： | 15168 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, single cells growing in suspension and some slightly adherent |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement") |
| Subculture ： | seed out at 0.3 x 106 cell/ml; maintain at 0.2-0.6 x 106 cells/ml; split saturated cultures 1:2 to 1:4 every 2-3 days; cells do not grow without HFCS, but it can be used at only 50% of the recommended (1:100) dilution; cell viability may not exceed 80% |
| Incubation ： | at 37°C with 10% CO2 |
| Doubling time ： | about 30-35 hours |
| Harvest ： | maximal density of about 0.6-0.8 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgG3 monoclonal antibody |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |