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| 产品名称： | MMQ |
|---|---|
| 商品货号： | TS259708 |
| <strong>Cell line </strong>： | MMQ |
| DSMZ no. ： | TS259708 |
| Species ： | confirmed as rat by cytogenetics and PCR analysis with Alu, globin and rRNA primers |
| Cell type ： | pituitary tumor |
| Origin ： | cell line is derived from the transplantable 7315a rat pituitary tumor; until establishment of the cell line, the 7315a pituitary tumor was serially transplanted in adult female Buffalo rats since the early 1960s; described as expressing prolactin and dopamine receptors; cells were reported to be responsive to somatostatin, estrogen, progesterone, and acetylcholin |
| Reference(s) ： | 14441, 14642 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | spheroidal cells growing in clumps in suspension |
| Medium ： | 90% RPMI 1640 + 7.5% horse serum + 2.5% h.i. FBS |
| Subculture ： | optimal split ratio of 1:2 to 1:4 every 2-4 days; seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-1.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | maximum density at 1.5-2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 15% horse serum, 5% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | rat hyperdiploid karyotype with 6% polyploidy - 49-52<2n> - high level of spontaneous breakage |
| Viruses ： | PCR: SMRV - |