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| 产品名称： | HH-16 cl.2/1 |
|---|---|
| 商品货号： | TS259709 |
| <strong>Cell line </strong>： | HH-16 cl.2/1 |
| DSMZ no. ： | TS259709 |
| Species ： | originally confirmed as rat by cytogenetics and by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | mammary fibrosarcoma |
| Origin ： | established from the ascitic fluid of a rat with a tumor which was produced by injecting cultured cells of a chemically-induced (dimethylbenzanthracene) mammary tumor; rat: strain Sprague-Dawley, female, 240 days old, adult; the original tumor was induced by single feeding of DMBA on day 45 after birth; sister cell line of HH-16 cl. 4 (DSM ACC 358); cells were described in the literature to produce invasive fibrocarcinomas in S.D. rats upon s.c. injection with remote metastases |
| Reference(s) ： | 14925, 14981, 14617, 14982 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblastoid growing in a criss-cross pattern |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | split confluent culture 1:4 every 3-4 days using trypsin/EDTA (NB: cells are sensitive to trypsin/EDTA and should not be incubated for more than 5 min at room temperature); seed out at ca. 1-2 x 106 cells/80 cm2; maintain at 4-5 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | cell harvest of ca. 7-8 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | rat near diploid karyotype with 11% polyploidy - 39-43<2n> |
| Viruses ： | PCR: SMRV - |