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| 产品名称： | HPB-ALL |
|---|---|
| 商品货号： | TS259714 |
| <strong>Cell line </strong>： | HPB-ALL |
| DSMZ no. ： | TS259714 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | T cell leukemia |
| Origin ： | established from the peripheral blood of a 14-year-old Japanese boy with ALL and thymoma at diagnosis in 1973. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14785, 15217, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorph cells growing singly or in clusters in suspension; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml; after thawing, we recommend to use initially 24-well-plates and 20% FBS; cell viability may be as low as 50% after thawing and cells may be be quiet for up to one week and then recovery begins slowly; for the first week do not dilute, only spent medium exchange is recommended; when growing exponentially split saturated culture 1:2 to 1:3 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | maximum density of about 4 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 +, CD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta +, TCRgamma/delta -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | pseudodiploid human karyotype with 8% polyploidy - 46<2n>XY, der(1)t(1;16)(q22;p11-12)add(16)(p13), del(2)(p24), del(3)(p11), der(5)t(1;5)(q22-24;q32-33), r(16)(?p12?q12) - sideline with -3 instead of del(3) - resembles published karyotype |
| Molec. Genetics ： | expression of TLX3 confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |