微信小李
微信小章
产品简介
购买须知
| 产品名称： | GH4-C1 |
|---|---|
| 商品货号： | TS259715 |
| <strong>Cell line </strong>： | GH4-C1 |
| DSMZ no. ： | TS259715 |
| Species ： | confirmed as rat by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | pituitary tumor |
| Origin ： | subclone of the cell line GH3 (ACC 469); established from a pituitary tumor induced in a female Wistar/Furth rat in 1965; described in the literature to produce a high rate of prolactin and a low rate of growth hormone synthesis |
| Reference(s) ： | 14880, 15008, 15009, 15004, 15194 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | mostly round cells growing singly and in clusters in suspension and partially adherent; quality and amount of serum may influence the adherence of the cells |
| Medium ： | 80-90% Hams F10 + 10-20% h.i. FBS + 2 mM L-glutamine (originally grown with 15% horse serum + 2.5% h.i. FBS in Ham´s F10) |
| Subculture ： | cells grow slowly and do not reach high cell densities; seed out at ca. 0.5 x 106 cells/ml; maintain at 0.3-0.8 x 106 cells/ml; adherent cells might be detached by "washing" or with TNE (40 mM TrisCl pH 7.5, 1 mM EDTA pH 7.5, 150 mM NaCl); |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-80 hours |
| Harvest ： | maximal density of 0.8 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with enrofloxacin (Baytril), then negative in microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | rat karyotype |
| Viruses ： | PCR: SMRV - |