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| 产品名称： | ACH1P |
|---|---|
| 商品货号： | TS259717 |
| <strong>Cell line </strong>： | ACH1P |
| DSMZ no. ： | TS259717 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | choriocarcinoma-trophoblast hybrid |
| Origin ： | established by somatic hybridization of primary male trophoblast cells and the HGPRT-defective choriocarcinoma cell line AC1-1 which was originally derived from cell line JEG-3 (DSM ACC 463) |
| Reference(s) ： | 14543 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial-like cells growing as monolayers, cells carry a large number of dark granula |
| Medium ： | 90% Hams F12 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:4 every 4-5 days week using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | cell harvest of ca. 8 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with ciprofloxacin, then negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Cytogenetics ： | human flat-moded hypertetraploid karyotype with 10% polyploidy - 85-104<4n>XXY/XXYY/XXXY, +2, +5, +7, -10, -11, +12, -13, +16, -18, +21, +1-4 mar, add(7)(q32), i(13q), add(15)(p11) add(19)(p13)x1-2 - sideline with del(X)(p11p22), der(X)(Xpter->Xq2?8::4p1? ->4p1?::11q1?1->11qter), der(4)t(4;11)(p14-16;q1?3), der(7)t(7;7)(p22;q11.22), add(11)(p15) - karyotype closely resembles AC-1M32/46/59/81/88 (daughters) and JEG-3 (parent) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |