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| 产品名称： | HCC-33 |
|---|---|
| 商品货号： | TS259719 |
| <strong>Cell line </strong>： | HCC-33 |
| DSMZ no. ： | TS259719 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | small cell lung carcinoma |
| Origin ： | established from the pleural effusion of a 52-year-old man with small cell lung carcinoma; matched EBV+ B-lymphoblastoid cell line (B-LCL) is available (HCC-33BL) |
| Reference(s) ： | 14567, 15046 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to ovoid cells growing singly or in clumps |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | split saturated culture 1:2 to 1:3 every 2-5 days; seed out at ca. 1.0 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; after thawing, cell clumps should be carefully resuspended to encourage growth |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | cell harvest of ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotriploid karyotype with 8% polyploidy - 61-68<3n>XX, -Y, +1, +1, +3, -10, -12, -13, +17, -20, -22, +3-5 mar, ider(1)(q10)del(1)(?q12?q43)x2, add(1)(p36), del(1)(p32)x1-2, dup(2)(p23p24), der(3;17)(q10;q10)x1-2, add(8)(p11), del(9)(q21), der(12)t(11;12)(q13.4;q24.3), add(13)p11) - sideline with add(5)(p11), add(15)(p11), add(17)(p1?), del(22)(q12) - variable dmin present in most cells - dup(2) includes MYCN locus |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |