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| 产品名称： | PR-1 |
|---|---|
| 商品货号： | TS259720 |
| <strong>Cell line </strong>： | PR-1 |
| DSMZ no. ： | TS259720 |
| Species ： | confirmed as rat by cytogenetics and species PCR |
| Cell type ： | pituitary tumor |
| Origin ： | established from the pituitary tumor of an ovarectomized F344 rat treated with estrogen for 3 months; described as showing markedly reduced levels of TGF-ß1 and TGF-ß type II receptor mRNA; reported as being resistant to antiproliferative effects of TGF-ß1 |
| Reference(s) ： | 14835 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells, slightly adherent, growing as strings of round cells in branched colonies |
| Medium ： | Dulbeccos MEM + Hams F12 (1:1) + 2.5% h.i. FBS + 10% horse serum |
| Subculture ： | optimal split ratio of 1:2 once a week using trypsin/EDTA; seed out at ca. 3 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 90 hours |
| Harvest ： | cell harvest of ca. 50 x 106 cells/80 cm2 |
| Storage ： | frozen with 65% medium (1:1), 5% FBS, 20% horse serum, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | rat near-triploid karyotype with 10% polyploidy - 60-68<3n> |
| Viruses ： | PCR: SMRV - |