微信小李
微信小章
产品简介
购买须知
| 产品名称： | NK-92 |
|---|---|
| 商品货号： | TS259724 |
| <strong>Cell line </strong>： | NK-92 |
| DSMZ no. ： | TS259724 |
| Species ： | confirmed originally as human by cytogenetics |
| Cell type ： | natural killer lymphoma |
| Origin ： | established from the peripheral blood of a 50-year-old man with non-Hodgkin lymphoma (large granular lymphocytic) in 1992; cells were described as having azurophilic granula and strong cytotoxic NK activity. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14487, 14570, 14725, 18187 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | cells growing in clumps in suspension; image; image; image |
| Medium ： | 75% alpha-MEM (with ribo- and deoxyribonucleosides) + 12.5% h.i. FBS + 12.5 % h.i. horse serum + 2 mM L-glutamine + 5 ng/ml IL-2 |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.2-0.6 x 106 cells/ml; split saturated culture 1:2 every 2 days; after thawing, it is recommended to first culture the cells in 24-well-plates; viability may not surpass 60-70%; cells grow mainly in large clusters, outside these clusters a significant amount of cell debris and dead cells are always visible |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | maximum density of about 0.8 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 +, CD3 -, CD4 -, CD5 -, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotetraploid karyotype with 4% polyploidy - 80(75-82)<4n>XXYY, +3, -4, -8, -9, -10, +12, +12, -14, -17, -17, -22, der(3;5)(q10;p10), i(7q), der(7)t(7;7)(p21;q11), der(8)t(8;8)(p23;q13), der(8)t(8;15)(p23.2;q14)x1-2, del(9)(p12p21), der(9)t(9;13)(p12;q11), der(10)t(10;18)(p12;q11)t(10;11)(q24;p13), der(12)t(11;12)(q13.4;q11)x1-2, der(13;19)(q10;q10), del(15)(q14)x1-2, del(16)(q23), der(16)t(11;16)(q22.2;q22), del(20)(?q12q13.1), der(Y)t(Y;17)(p11.3;q11), der(Y)t(Y;17)(p11.3;q11)t(11;17)(p1?2;q25) - sideline with der(9;21)(p10;q10) |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |