微信小李
微信小章
产品简介
购买须知
| 产品名称： | MUTZ-5 |
|---|---|
| 商品货号： | TS259726 |
| <strong>Cell line </strong>： | MUTZ-5 |
| DSMZ no. ： | TS259726 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established from the peripheral blood of a 26-year-old man with B cell precursor acute lymphoblastic leukemia (BCP-ALL) at relapse in 1998; simultaneous sister cell lines MUTZ-6 and MUTZ-7 |
| Reference(s) ： | 14762 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | cells grow singly or in small clusters in suspension, irregular cell forms; image; image |
| Medium ： | 80% McCoys 5A + 20% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 1 x 106 cells/ml; after thawing, culturing in 24-well-plate is of advantage; split saturated culture 1:2 to 1:3 every 2-4 days; minimum cell density of 0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70-80 hours |
| Harvest ： | maximum density at 3.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD37 +, CD38 +, CD71 +, CD80 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype - 45<2n>X, -Y, t(12;13)(p12;q13-14) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |