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| 产品名称： | SU-DHL-4 |
|---|---|
| 商品货号： | TS259729 |
| <strong>Cell line </strong>： | SU-DHL-4 |
| DSMZ no. ： | TS259729 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the peritoneal effusion of a 38-year-old man with B-NHL (diffuse large cell, cleaved cell type; originally described as "diffuse histiocytic lymphoma") in 1975; cell line carries EZH2 Y641S mutation; assigned to GCB-like lymphoma subtype (germinal center B-cell). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14514, 14515, 14516, 14605, 15065, 16554, 17354, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells growing in suspension; image; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1.0 x 106 cells/ml; after thawing, during the first week cells should be kept with 20% FBS; culture in 12- or 24-well-plate is of advantage; split saturated culture 1:2 to 1:3 every 2-3 days; maintain at about 0.5-1.5 x 106 cells/ml; maximum cell density of about 2.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | cell harvest of about 1.0-2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 4% polyploidy - 50(47-51)<2n>XY/XXY, -3, +7, +8, +12, +13, -16, +18, der(3)t(3;3)(p2?4;p1?2), der(8)t(3;8)(p1?2;q12)?inv(3)(p1?p2?), der(12)t(3;12)(p1?;q24)add(3)(p2?4), add(13)(p11), t(14;18)(q32.3;q21.1), add(16)(p13), der(18)t(14;18)(q32.3;q21.1) - carries t(14;18) effecting BCL2(MBR)-IGH fusion - matches published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |