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| 产品名称： | HDQ-P1 |
|---|---|
| 商品货号： | TS259732 |
| <strong>Cell line </strong>： | HDQ-P1 |
| DSMZ no. ： | TS259732 |
| Species ： | originally confirmed as human by cytogenetics |
| Cell type ： | breast carcinoma |
| Origin ： | established in 1995 from a primary ductal infiltrating breast carcinoma of a 50-year-old Caucasian woman; cells are described in the literature to be estrogen- and progesterone-receptor negative, and to possess a point mutation in p53 and a five-fold amplification of MYC |
| Reference(s) ： | 15052 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial cells growing adherently in monolayers; image; image ; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:2 to 1:3 every 4-5 days using trypsin/EDTA (wash adherent cells twice with PBS) or apply "Accutase" |
| Incubation ： | at 37 °C with 5-10% CO2 |
| Doubling time ： | ca. 72 hours |
| Harvest ： | cell harvest of ca. 4 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human highly-rearranged flat-moded hypertetraploid/hypopentaploid karyotype with 13% polyploidy - 92-107<4n>XX, -X, -X, +1, -3, -3, -3, +5, +5, +5, +6, +7, +7, -8, +10, -13, -13, -14, -15, -15, -16, -16, -17, -17, -18, -18, -18, -19, -19, -20, -20, -21, -21, -22, -22, -22, +28-29mar, add(X)(p2?)x2, add(1)(p36)x2, add(1)(p35), add(1)(q1?2)x2, der(2)t(2;3)(p16;q11), add(5)(p11q3?4)x2, add(5)(q11), der(5)t(5;?;3)(q1;?;p11), der(5)t(5;5)(p1?;q1?4), i(5p), add(6)(p11), add(7)(p14), add(7)(p11)x2, add(7)(q11)x2, add(8)(q21), del(9)(p23), del(9)(p11), i(10p), del(11)(q22), der(12)t(7;12)(q11;q24), add(14)(p11)x2, add(15)(p11), add(16)(p13), add(16)(q23), add(17)(p11), del(17)(p12), add(18)(p11), add(19)(p13), add(19)(q13), del(22)(q12.2) - HDQ-P1 markers |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |