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| 产品名称： | HCC-366 |
|---|---|
| 商品货号： | TS259735 |
| <strong>Cell line </strong>： | HCC-366 |
| DSMZ no. ： | TS259735 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | non-small cell lung carcinoma |
| Origin ： | established from the tumor of an 80-year-old woman with non-small cell lung carcinoma (subtype adeno-squamous carcinoma); cells were described in the literature to carry fractional allellic loss with loss of heterozygosity at various hot spots; matched EBV+ B-lymphoblastoid cell line (B-LCL) is available (HCC-366BL) |
| Reference(s) ： | 14567, 14821, 15046 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epitheloid cells growing as monolayer; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca.0.5-1 x 106 cells/80 cm2; split confluent culture 1:2 to 1:3 once a week using trypsin/EDTA; maximal density at ca. 2 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 72-96 hours |
| Harvest ： | cell harvest of ca. 4 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hyperdiploid karyotype with 12% polyploidy - 48-56<2n>XX, +1, +1, -2, +4, +7, +8, +11, -13, -15, -18, +7-9mar, add(X)(p11), add(1)(p22), add(1)(q11)x1-2, add(4)(q31)x1-2, del(8)(p12), del(8)(q13), add(10)(p13), add(14)(p11), add(17)(p12) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |