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| 产品名称： | KCI-MOH1 |
|---|---|
| 商品货号： | TS259738 |
| <strong>Cell line </strong>： | KCI-MOH1 |
| DSMZ no. ： | TS259738 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | pancreas adenocarcinoma (derivative of HPAC) |
| Origin ： | originally described in the literature to be derived from the moderately differentiated adenocarcinoma of the head of the pancreas from a 74-year-old African-American man in 1996; patient was treated with pancreato-duodenectomy; cells were described as being tumorigenic in SCID mice; however, DNA fingerprinting and cytogenetic analysis showed cross-contamination with cell line HPAC which was established in 1985 from the pancreas adenocarcinoma of a 64-year-old Caucasian woman |
| Reference(s) ： | 14776 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial-like cells growing in monolayers |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:2 to 1:3 every 3-4 days using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm2; maintain at ca. 3-4 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70 hours |
| Harvest ： | maximal density at ca. 7 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with enrofloxacin (Baytril), then negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA profile as HPAC by fluorescent nonaplex PCR of short tandem repeat markers |
| Cytogenetics ： | human hypotriploid karyotype with 8% polyploidy - 61(57-63)<3n>X/XX, -Y, +1, -2, -6, +8, -10, +11, -12, -13, -14, -16, -18, -21, -21, +mar, add(8)(p2 ?)x2, i(11p), del(11)(p12), add(13)(p11), add(17)(p12) - differs from published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |