微信小李
微信小章
产品简介
购买须知
| 产品名称： | MEC-1 |
|---|---|
| 商品货号： | TS259741 |
| <strong>Cell line </strong>： | MEC-1 |
| DSMZ no. ： | TS259741 |
| Species ： | confirmed originally as human by cytogenetics |
| Cell type ： | chronic B cell leukemia |
| Origin ： | established in 1993 from the peripheral blood of a 61-year-old Caucasian man with chronic B cell leukemia (B-CLL in prolymphocytoid transformation to B-PLL); serial sister cell line of MEC-2 (DSMZ ACC 500). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14980, 18187 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Commission for Biosafety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used for genetic manipulations. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorphic cells growing in suspension, singly or partly in small aggregates, a few cells are slightly adherent; image |
| Medium ： | 90% Iscoves MDM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; after thawing, during the first week cells should be kept with 20% FBS initially; culture in 24-well-plate is of advantage; maintain at about 0.5-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | cell harvest of about 2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 +, HLA-DR +; image |
| Fingerprint ： | same DNA profile as MEC-2 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human near-diploid karyotype with 10% polyploidy - 46(44-47)<2n>XY, -2, +7, -12, +1-2mar, t(1;6)(q22-23;p21), add(7)(q11), der(10)(10pter->q22::?::2q11->qter), del(17)(p11) - small acf/mar present in most cells - resembles published karyotype |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |