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| 产品名称： | H-209 |
|---|---|
| 商品货号： | TS259745 |
| <strong>Cell line </strong>： | H-209 |
| DSMZ no. ： | TS259745 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | small cell lung carcinoma |
| Origin ： | established in 1979 from the bone marrow aspirated from a 55-year-old white man with small cell lung carcinoma prior to treatment; corresponds to NCI-H209; described as expressing neuroendocrine biochemical markers, e.g. neuron-specific enolase, brain creatine kinase, L-DOPA decarboxylase and bombesin |
| Reference(s) ： | 14421, 14849 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | spherical-to-ovoid cells growing singly in large clumps |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | split saturated culture 1:2 to 1:3 every 2-4 days; seed out at ca. 0.7-1.0 x 106 cells/ml; maintain at ca. 0.5-1.5 x 106 cells/ml; note that cells grow slowly after thawing; appearing clumps may be resuspended in original medium to encourage growth |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-70 hours |
| Harvest ： | maximum density of ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 8% polyploidy - 47(44-48)<2n>XY, -4, -5, +6, +7, +8, -10, -14, -15, -17, +0-2mar, r/dm, add(1)(p32-34), add(3)(p11), add(4)(q24-25), add(5)(p1?), add(6)(q14)x1-2, add(8)(p1?), add(17)(p1?) - extensive subclonal variation |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |