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| 产品名称： | HCC-15 |
|---|---|
| 商品货号： | TS259747 |
| <strong>Cell line </strong>： | HCC-15 |
| DSMZ no. ： | TS259747 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | non-small cell lung carcinoma |
| Origin ： | established from the tumor of a 47-year-old man with non-small cell lung carcinoma (subtype squamous carcinoma); matched EBV+ B-lymphoblastoid cell line (B-LCL) is available (HCC-15BL) |
| Reference(s) ： | 14567, 15046 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epitheloid cells growing as monolayer; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:4 to 1:6 every 4-5 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | cell harvest of ca. 8 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 16% polyploidy - 37(34-38)<2n>X, -X, -4, -13, -14, -17, -18, -19, -20, -22, +1-2mar, del(3)(q13), der(5;19)(q10;q10)add(19)(q13), add(9)(p2?), del(10)(p12-13), add(12)(q24.3), del(20)(?p12), add(22)(q1?) - sideline with add(8)(p12), del(7)(q21) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |