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| 产品名称： | CL-14 |
|---|---|
| 商品货号： | TS259751 |
| <strong>Cell line </strong>： | CL-14 |
| DSMZ no. ： | TS259751 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | colon carcinoma |
| Origin ： | established from a male patient with a well-differentiated primary sigmoid adenocarcinoma of TNM stage T3N0M0 |
| Reference(s) ： | 14528 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | strongly adherent epithelioid cells growing in monolayers and forming islets; image; image |
| Medium ： | 80% mixture of Dulbeccos MEM + Hams F12 at 1:1 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/25 cm2; maintain at ca. 1-2 x 106 cells/25 cm2; split confluent culture 1:2 once a week; cells are difficult to detach, it is recommended to use trypsin/EDTA at higher concentration or "Accutase" (PAA, Austria) and to incubate for 20-30 min at 37 °C, avoid any scraping; reduced viability due to long exposure to trypsin |
| Incubation ： | at 37 °C with 5-10% CO2 |
| Doubling time ： | ca. 1 week |
| Harvest ： | cell harvest of ca. 2 x 106 cells/25 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotetraploid karyotype with 3.5% polyploidy - 78-85<4n>XX, -Y, -Y, -3, -3, -4, -4, +5, +5, +6, -8, +9, +9, -10, -10, +11, -13, -14, -16, -16, -17, -18, -18, -18, -21, -21, -21, -22, +6-12mar, del(1)(p32)x2, i(5p)x3, add(14)(p11)x2, add(17)(p11), add(19)(q13)x2 - ring and large isodicentric hsr marker chromosomes present |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, MLV -, SMRV - |