微信小李
微信小章
产品简介
购买须知
| 产品名称： | JMSU-1 |
|---|---|
| 商品货号： | TS259754 |
| <strong>Cell line </strong>： | JMSU-1 |
| DSMZ no. ： | TS259754 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | urinary bladder carcinoma |
| Origin ： | derived from malignant ascitic fluid of a 75-year-old man with urinary bladder carcinoma in 1988; described as being of epithelial origin with morphologically distinct cells (polygonal to spindle-shaped) and as expressing cytokeratin-19 fragment |
| Reference(s) ： | 14786, 14787 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | longly cells, some forming fiber-like contacts, growing adherently in monolayers and small clusters; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:2 to 1:4 every 2-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypertriploid karyotype with 12% polyploidy - 69-74<3n>X/XX, -Y, -Y, +1, +1, +2, -5, +9, -12, +13, -15, -15, -16, -17, +19, -22, +9mar, add(1)(q32), add(2)(q3?), del(4)(q31q33), del(6)(q25), add(7)(p11), del(8)(p22), del(9)(p22), del(10)(p12), del(10)(q22), add(11)(p12), add(13)(p11), add(16)(p13), i(17q), add(19)(p13) - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |