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| 产品名称： | H-1184 |
|---|---|
| 商品货号： | TS259756 |
| <strong>Cell line </strong>： | H-1184 |
| DSMZ no. ： | TS259756 |
| Species ： | confirmed originally as human by cytogenetics |
| Cell type ： | small cell lung carcinoma |
| Origin ： | established in 1985 from the lymph node from a 42-year-old white man with metastatic small cell lung carcinoma prior to treatment; corresponds to NCI-H1184; matched B-lymphoblastoid cell line (B-LCL) is available |
| Reference(s) ： | 14849, 14421 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small epitheloid cells growing adherently in monolayers or in suspension as clusters |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/cm2 in a 80 cm2 flask; split saturated culture 1:2 to 1:4 every 5-8 days using trypsin/EDTA; after recovery change medium sparingly |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-70 hours |
| Harvest ： | maximum density of ca. 20-25 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 8% polyploidy - 57(53-59)<2n>XY/XXY, +1, +3, +5, +8, +9, -10, -10, +11, +12, -14, +16, +20, +22, +6mar, add(1)(p34), add(1)(p11), add(3)(p14), add(3)(q21-22), add(5)(p11), add(5)(q11), add(8)(q21), add(11)(q25), add(12)(q24)x1-2, der(12)t(11;12)(q11;q24), add(13)(p11), del(?13)(q12q21), del(16)(p12p13), der(?22)t(14;22)(q21;q13) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |