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| 产品名称： | TT2609-C02 |
|---|---|
| 商品货号： | TS259761 |
| <strong>Cell line </strong>： | TT2609-C02 |
| DSMZ no. ： | TS259761 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | follicular thyroid carcinoma |
| Origin ： | established in 1996 from the tumor tissue of a 57-year-old Caucasian man with local recurrence of a previously iodine-irradiated follicular thyroid cancer (well-differentiated with dispersed poorly differentiated areas). Cells express the transcriptionally inactive delta p53 isoform. A sister cell line with a truncated delta p53 -/- is also available (ACC 859). Useful for studies with p53 family members p63 and p73 |
| Reference(s) ： | 14552 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small fibroblastoid cells growing adherent but irregularly in monolayers |
| Medium ： | 80-85% RPMI 1640 + 15-20% h.i. FBS + 1 mM sodium pyruvate + insulin-transferrin-selenite medium supplement |
| Subculture ： | split confluent culture 1:3 to 1:4 every week using trypsin/EDTA (for 10 min); seed out at ca. 1 x 106 cells/25 cm2; maintain at ca. 8 x 106 cells/175 cm2; maximal density at about 14 x 106 cells/175 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-80 hours |
| Harvest ： | cell harvest of about 10 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed an identical STR DNA profile to sister cell line TT2609-C02 (ACC 859) |
| Cytogenetics ： | human flat-moded hypertriploid karyotype with 4% polyploidy - 69-75<3n>XXYY, -2, +8, +8, +9, +10, +14, -15, -16, +17, -22, +1-2mar, der(1)t(1;2)(p32;q11-13), add(4)(p15.2), ?inv(8)(q12q21.2)x1-2, add(9)(p24)x2, i(15q), del(17)(p11)x1-2, add(22)(p11)x2 - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |