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| 产品名称： | SUP-M2 |
|---|---|
| 商品货号： | TS259764 |
| <strong>Cell line </strong>： | SUP-M2 |
| DSMZ no. ： | TS259764 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | anaplastic large cell lymphoma |
| Origin ： | derived from the cerebrospinal fluid of a 5-year-old girl with refractory malignant histiocytosis; described in the literature to carry the typical t(2;5)(p23;q35) leading to the NPM1-ALK (NPM-ALK) fusion gene and to express CD30, defining the cells as anaplastic large cell lymphoma (ALCL). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14392, 14782, 14737, 14492, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly or in clumps in suspension; image ; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.3-0.5 x 106 cells/ml; first week after thawing cultivate in 24-well-plate, initially cells grow slowly and with a reduced viability; later split saturated culture 1:3 to 1:5 every second day; maintain at ca. 0.5-1.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | maximal cell harvest of about 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD2 +, CD3 -, CD4 +, CD13 -, CD14 -, CD19 -, CD21 -, CD25 +, CD30 +, CD34 -, CD71 +, HLA-DR +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded near-diploid karyotype with 3.5% polyploidy - 44-50<2n>XX/XXX, +1, +7, +8, add(X)(q26), der(X;7)(q10;p10), add(1)(q21), der(1)(1pter->q43::1q43->q21::9q31->qter), t(2;5)(p23;q35), der(6)t(6;6)(q27;p11), add(9)(q31), add(11)(q24)/der(11)t(4;11)(?q34;q24), del(19)(q13.2) - extensive subclonal changes incl. dicentric t(1;1) - carries t(2;5) effecting NPM1-ALK (NPM-ALK) fusion - resembles published karyotype |
| Molec. Genetics ： | expression of fusion gene NPM1-ALK (NPM-ALK) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, MLV -, SMRV - |