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| 产品名称： | ONCO-DG-1 |
|---|---|
| 商品货号： | TS259767 |
| <strong>Cell line </strong>： | ONCO-DG-1 |
| DSMZ no. ： | TS259767 |
| Species ： | originally confirmed as human by cytogenetics |
| Cell type ： | ovary adenocarcinoma (derivative of OVCAR-3) |
| Origin ： | established from the malignant tumor of a 49-year-old woman with well-differentiated oxyphilic papillary thyroid carcinoma (PT2, G1, No, Mo); cells were described in the literature to be tumorigenic in nude mice; however, DNA fingerprinting analysis at the DSMZ showed cross-contamination with cell line OVCAR-3; OVCAR-3 line was established from the malignant ascites of a patient with progressive adenocarcinoma of the ovary |
| Reference(s) ： | 14578, 18203 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like adherent cells with large vacuoles growing as monolayers; image ;image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:5 every 3-4 days using trypsin/EDTA (for 10 min) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of about 10 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with ciprofloxacin (Ciprobay), then negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed same DNA profile as OVCAR-3 |
| Cytogenetics ： | human hypotriploid karyotype with 8% polyploidy - 59(56-61)<3n>XX, -X, +1, -2, -4, -6, -8, -9, -13, -13, -14, -15, -15, -17, -17, -18, -19, -21, -22, +7-9mar, +0-3 dm, add(1)(p3?5), add(1)(p2?1), add(1)(q11), der(3;15)(p10;q10), add(3)(p14), del(5)(q23), add(6)(q23), der(7;14)(q10;q10), del(9)(p23), add(10)(p11), add(11)(p11)x2, del(?11)(q13q14.2), der(12;18)(q10;q10), add(13)(p11), add(14)(p11), add(16)(q23-24), add(17)(p11), add(21)(p11)x1-2 - carries large submetacentric hsr marker |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |