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| 产品名称： | HCC-1143 |
|---|---|
| 商品货号： | TS259768 |
| <strong>Cell line </strong>： | HCC-1143 |
| DSMZ no. ： | TS259768 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | breast carcinoma |
| Origin ： | established from a primary, invasive, ductal breast carcinoma (TNM stage IIa, tumor size 5, grade 3, without lymph node metastasis) from a 52-year-old white woman prior to therapy in 1994; a paired EBV+ B-lymphoblastoid cell line (B-LCL HCC-1143BL) is available; reported to express EGP2, CK19 buth neither estrogen nor progesterone receptors are expressed |
| Reference(s) ： | 14550 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing as adherent monolayers; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | split confluent culture 1:2 to 1:3 every 4-7 days using trypsin/EDTA (for 10-20 min); seed out at ca. 1.0-1.7 x 106 cells/80 cm2 flask |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-60 hours |
| Harvest ： | cell harvest of about 0.4-0.9 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human highly rearranged flat-moded hypertriploid karyotype with 8% polyploidy - 74-78<3n>XXX, -2, +3, -4, -6, +7, +7, +7, -9, -10, -11, -11, -13, -15, -17, -19, +20, +14-20 mar, +2 dmin, add(X)(p11.3), add(X)(p22)add(X)(q11), add(X)(q25-27), del(1)(p22), del(1)(q13)x1-2, del(3)(q12)x2, del(4)(q11), add(4)(p15), del(5)(q13), der(6)t(1;6)(q22;q24), add(7)(p14), del(7)(p14), del(7)(p11), add(7)(p11), ins(7;?X)(q11;?), add(7)(q3?), del(8)(p1?), del(9)(p22), add(11)(q23), add(12)(q24), add(13)(p11), der(14;21)(q10;q10), add(14)(p11), add(15)(p11), add(15)(p11), add(16)(q24), i(18q), add(22)(p11) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |