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| 产品名称： | HCC-1937 |
|---|---|
| 商品货号： | TS259772 |
| <strong>Cell line </strong>： | HCC-1937 |
| DSMZ no. ： | TS259772 |
| Species ： | human (Homo sapiens) |
| Cell type ： | breast carcinoma |
| Origin ： | established from a primary ductal carcinoma of the breast mammary gland from a 24-year-old Caucasian woman in 1995; cells were described in the literature to be homozygous for the BRCA1 mutation; a paired EBV+ B-lymphoblastoid cell line (B-LCL HCC-1937BL) is available |
| Reference(s) ： | 15019 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial cells growing as monolayer, about 5-10% are giant cells; image |
| Medium ： | 85-90% RPMI 1640 + 10-15% h.i. FBS |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:4 every 2-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of about 5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Molec. Genetics ： | expression of fusion gene NFIA-EHF was confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |