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| 产品名称： | ARH-77 |
|---|---|
| 商品货号： | TS259775 |
| <strong>Cell line </strong>： | ARH-77 |
| DSMZ no. ： | TS259775 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | plasma cell leukemia |
| Origin ： | established from the peripheral blood of a 33-year-old woman with IgG plasma cell leukemia; cell line is widely used as model for multiple myeloma/plasma cell leukemia, but cells are clearly EBV+ indicating that ARH-77 might be rather a "normal" B-lymphoblastoid cell line than a tumor cell line |
| Reference(s) ： | 14844, 14471, 14472, 14486 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round single cells or clustered growing in suspension, some cells loosely adherent; image |
| Medium ： | 90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | split saturated culture 1:2 to 1:5 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml; start culture in 24-well-plates; maintain at 0.2-1.5 x 106 cells/ml; adherent cells can be detached by tapping the flask |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | saturation density at ca. 1.5-2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with enrofloxacin (Baytril), then negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD71 +, cyCD79 +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near diploid karyotype with 8% polyploidy - 46(44-48)<2n>XX, +9, del(1)(q23), add(2)(q21), add(3)(p11), der(3)t(2;3)(q23;q26), del(6)(p21), der(9)t(9;17)(q10;q10) - sideline with der(X)t(X;1)(q23;p32), del(16)(p13.2) and unresolved der(3) and der(9)hsr markers - no IGH translocations detected |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |