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| 产品名称： | ALL-SIL |
|---|---|
| 商品货号： | TS259777 |
| <strong>Cell line </strong>： | ALL-SIL |
| DSMZ no. ： | TS259777 |
| Species ： | confirmed as human by cytogenetics and DNA fingerprint |
| Cell type ： | T cell leukemia |
| Origin ： | established from the peripheral blood of a 17-year-old man with T-ALL (T cell acute lymphoblastic leukemia) at relapse; also found in the literature as SIL-ALL; carries NUP214-ABL1 fusion gene |
| Reference(s) ： | 14489, 14574, 15217 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | mostly round cells growing singly or in small clusters in suspension; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days; maintain at ca. 0.5-1.5 x 106 cells/ml; maximal density of ca. 1.5 x 106 cells/ml; it may be of advantange to first culture the cells after thawing in a 24-well-plate with 20% FBS |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | cell harvest of about 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with ciprofloxacin (Ciprobay), then negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | cyCD3 +, CD3 -, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertetraploid karyotype - 90-95<4n>XX/XXYY, +6, +8, +8, t(1;13)(p32;q32)x2, del(6)(q25)x2, del(9)(?p23p24)x2, t(10;14)(q24;q11.2)x2, add(17)(p11)x2 - sideline with idem, -20, -20 - carries t(10;14) effecting TLX1-TRD (HOX11-TCRD) juxtaposition - matches published karyotype |
| Molec. Genetics ： | expression of TLX1 confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |