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| 产品名称： | NALM-19 |
|---|---|
| 商品货号： | TS259783 |
| <strong>Cell line </strong>： | NALM-19 |
| DSMZ no. ： | TS259783 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established from the peripheral blood of a 26-year-old man with "acute undifferentiated leukemia" at diagnosis in 1988 |
| Reference(s) ： | 14741, 14744 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small single round cells growing in suspension, sometimes in small clumps; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | cells are difficult to culture; seed out at ca. 3 x 106 cells/ml and culture in 24-well-plates all the time; the first 1-3 weeks after thawing cells do not grow or only very slowly (exchange spent medium without dilution); in growth phase, split saturated culture 1:2 every 2-3 days but do not dilute below 2 x 106 cells/ml; maximal density of ca. 4 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-70 hours |
| Harvest ： | cell harvest of ca. 2-4 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD15 +, CD19 +, CD20 -, CD34 -, CD38 +, cyCD79a +, CD80 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, smkappa -, smlambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 4% polyploidy - 47(45-48)<2n>XY,+5, del(9)(p21.2) - revision of published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |