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| 产品名称： | SK-ES-1 |
|---|---|
| 商品货号： | TS259786 |
| <strong>Cell line </strong>： | SK-ES-1 |
| DSMZ no. ： | TS259786 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | Ewings sarcoma |
| Origin ： | established from the Ewing`s sarcoma of an 18-year-old man in 1971; described in the literature to lack expression of HLA-ABC and E, but HLA-ABC can be reconstituted by TNF-alpha and IFN-gamma; cells are sensitive to adenovirus infection and express high levels of growth/differentiation factor midkine |
| Reference(s) ： | 14394, 14399, 14344 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent fibroblastoid cells growing as monolayer, cells do not become confluent (max. ca 25% confluent), formation of dense clumps which become larger |
| Medium ： | 80-90% McCoys 5A + 10-20% h.i. FBS |
| Subculture ： | split culture 1:3 to 1:5 twice a week using trypsin/EDTA; seed out at ca. 2-4 x 106 cells/80 cm2 flask; maximal density at ca. 2-3 x 105 cells/cm2; use new flask after splitting; clumps can be dispersed with trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35 hours |
| Harvest ： | cell harvest of about 30-40 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hyperdiploid karyotype - 49(46-51)<2n>XY, +7, +2-4mar, add(Y)(q12), add(3)(q2?7), del(3)q13), add(8)(p11), ?idic(9)(q3?4.2)del(9) (q1?q2?)add(9)(p11), t(11;22)(q24;q12), add(12)(p13), del(16)(q21) - carries t(11;22) effecting EWS-FLI1 fusion yielding longer type II RT-PCR transcript |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |