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| 产品名称： | SEWA |
|---|---|
| 商品货号： | TS259791 |
| <strong>Cell line </strong>： | SEWA |
| DSMZ no. ： | TS259791 |
| Species ： | confirmed as mouse by cytogenetics and recently as mus musculus by COI DNA barcoding |
| Cell type ： | osteogenic sarcoma |
| Origin ： | established from a polyoma virus-induced osteogenic sarcoma in an A.SW mouse; cells were converted into ascitic form growing in suspension by serial intraperitoneal inoculation into genetically compatible mice; described in the literature to have lost double minute chromosomes which were still present in the original tumor cell line, but reoccur when retransplanted into mice |
| Reference(s) ： | 15158, 14715 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly or in clumps in suspension |
| Medium ： | 90-95% RPMI 1640 + 5-10% h.i. FBS |
| Subculture ： | split saturated culture 1:4 to 1:10 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.3-1.5 x 106 cells/ml; maximal density of ca. 2.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 20 hours |
| Harvest ： | cell harvest of ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Cytogenetics ： | murine flat-moded near-diploid karyotype - 36-44<2n> - dmin rare or absent |
| Viruses ： | PCR: SMRV - |