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| 产品名称： | DERL-7 |
|---|---|
| 商品货号： | TS259792 |
| <strong>Cell line </strong>： | DERL-7 |
| DSMZ no. ： | TS259792 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | T cell lymphoma |
| Origin ： | established from the bone marrow blood of a 30-year-old Caucasian man with hepatosplenic gamma-delta T cell lymphoma (T-cell NHL) in 1995 at disease progression; simultaneous sister cell line of cell line DERL-2. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14746, 14416, 14460, 15237, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small ovoid ("lymphoblastic") cells growing singly or in small clumps in suspension; image ; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 20 ng/ml human IL-2 |
| Subculture ： | seed out at ca. 0.5-1.0 x 106 cells/ml in 12-well-plates for optimal growth, cultures may be continued in flasks after 2-3 weeks following recovery; split saturated culture 1:2 to 1:3 every 2-4 days; cultures may contain significant amounts of cell debris; maximal density of ca. 3.0 x 106 cells/ml; CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture and PCR assays |
| Immunology ： | CD2 +, CD3 -, CD4 -, CD5 -, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, HLA-DR -, TCRalpha/beta -, TCRgamma/delta -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile (identical to that of sister cell line DERL-2) |
| Cytogenetics ： | human hyperdiploid karyotype - 47(46-47)<2n>XY, +8, i(7q), add(10)(p12.1) - sideline with der(1)t(1;16)(q12.2;p13.3) - i(7q) is associated with hepatosplenic T-NHL - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |