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| 产品名称： | NTERA-2 |
|---|---|
| 商品货号： | TS259793 |
| <strong>Cell line </strong>： | NTERA-2 |
| DSMZ no. ： | TS259793 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | embryonal carcinoma (teratocarcinoma) |
| Origin ： | NTERA-2 was cloned from cell line TERA-2 which was derived from a metastatic teratocarcinoma of a 22-year-old Caucasian male; cell line also known as NT-2 |
| Reference(s) ： | 14358, 15130, 15135 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epitheloid cells carrying strong granula growing adherent in monolayer |
| Medium ： | 85% Dulbeccos MEM + 10% h.i. FBS + 5% horse serum |
| Subculture ： | split confluent culture 1:3 to 1:6 1-2 times a week using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm2; after thawing use collagen-coated flasks for the first week |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | cell harvest of about 14 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hyperdiploid karyotype - 48-58<2n>XXXY, +1, +16, +20, +22, +3-4mar, add(X)(p11), add(X)(q2?5), der(X;1)(p10;q10), add(1)(p35-36), add(11)(p15), add(13)(q3?1) - sideline with i(3q) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |