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| 产品名称： | DERL-2 |
|---|---|
| 商品货号： | TS259803 |
| <strong>Cell line </strong>： | DERL-2 |
| DSMZ no. ： | TS259803 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | T cell lymphoma |
| Origin ： | established from the peripheral blood of a 30-year-old Caucasian man with hepatosplenic gamma-delta T cell lymphoma (T-cell NHL) in 1995 at disease progression; simultaneous sister cell line of cell line DERL-7 |
| Reference(s) ： | 14416, 14460, 14746, 15237 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small multiformed cells growing in clusters in suspension; image; image ; image ; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 20 ng/ml human IL-2 |
| Subculture ： | seed out at ca. 1.0 x 106 cells/ml, cells grow slowly at first, cultivate in 6- or 12-well-plates for optimal growth; a significant amount of cell debris may be present in the culture all the time; split saturated culture 1:2 every 2-3 days; maximal density of ca. 2.5 x 106 cells/ml; CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60 hours |
| Harvest ： | cell harvest of ca. 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture and PCR assays |
| Immunology ： | CD2 +, CD3 -, CD4 -, CD5 -, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile (identical to that of sister cell line DERL-7) |
| Cytogenetics ： | human hyperdiploid karyotype with 8% polyploidy - 48(46-49)<2n>XY, +7, +8, +17, t(1;5)(p13;q32), i(7q), der(10;?17)(q10;q10) - sideline with +del(7)(p11) - i(7q) is associated with hepatosplenic T-NHL - matches originators karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |