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| 产品名称： | OCI-M1 |
|---|---|
| 商品货号： | TS259804 |
| <strong>Cell line </strong>： | OCI-M1 |
| DSMZ no. ： | TS259804 |
| Species ： | human (Homo sapiens) |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from erythroleukemia blasts (AML M6) of a 62-year-old patient following a 7-year chlorambucil treatment for chronic lymphocytic leukemia (CLL); described in the literature to express many markers of the erythroid lineage as well as several non-erythroid markers |
| Reference(s) ： | 15026, 14834, 14611, 14833 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly in suspension; image; image |
| Medium ： | 80-90% Iscoves MDM + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-0.5 x 106 cells/ml; split saturated culture 1:2 to 1:4 every second day; maximal density of ca. 1.5 x 106 cells/ml; immediately after thawing viability may be between 70 and 80% |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 1-1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 -, CD15 +, CD19 -, CD33 +, CD34 -, CD41 -, CD42 -, CD71 +, HLA-DR +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |