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| 产品名称： | JURL-MK1 |
|---|---|
| 商品货号： | TS259806 |
| <strong>Cell line </strong>： | JURL-MK1 |
| DSMZ no. ： | TS259806 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | chronic myeloid leukemia in blast crisis |
| Origin ： | established from the peripheral blood of a 73-year-old man with chronic myeloid leukemia (CML) in blast crisis in 1993; cells carry the Philadelphia chromosome; JURL-MK1 and JURL-MK2 are sister cell lines derived from the same patient |
| Reference(s) ： | 14459 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly or in small clusters in suspension; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split saturated culture 1:2 to 1:3 every 2-4 days; seed out at ca. 0.5-1.0 x 106 cells/ml; maintain at ca. 0.2-2.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | cell harvest of ca. 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 -, CD13 +, CD15 +, CD33 +, CD34 +, CD41 +, CD42 +, CD71 +, CD116 +, CD117 +, HLA-DR + |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile, sister cell line of JURL-MK2 |
| Cytogenetics ： | human hypodiploid karyotype with 4% polyploidy - 45(41-45)<2n>XY, -9, add(1)(p35), der(9)del(9)(p21.2)t(9;22)(q34;q11), der(11)t(11;9;22)(p15;q34;q11), add(12)(p13)x2, add(17)(p13), der(22)t(9;22)(q34;q11) - carries t(9;22) effecting BCR-ABL1 fusion (two copies) - does not appear to match published karyotype |
| Molec. Genetics ： | expression of fusion gene BCR-ABL1 e14-a2 (b3-a2) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |