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| 产品名称： | CL-40 |
|---|---|
| 商品货号： | TS259810 |
| <strong>Cell line </strong>： | CL-40 |
| DSMZ no. ： | TS259810 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | colon carcinoma |
| Origin ： | established from the primary colorectal cancer (TNM stage 3) of the right colon of a woman; cells were described in the literature to be well differentiated |
| Reference(s) ： | 14528 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial-like cells growing as monolayers and in aggregates; image; image |
| Medium ： | 80% mixture of Dulbeccos MEM + Hams F12 at 1:1 + 20% h.i. FBS |
| Subculture ： | split confluent culture 1:4 once a week using the double amount of trypsin/EDTA for up to 20 min (cells are difficult to detach), after detachment clumps of cells appear; to avoid multiple detachments cells can be seeded out at 2-3 x 106 cells/175 cm2; reduced viability immediately after thawing (ca. 50%), cells recover subsequently over the next weeks; maximal density of ca. 12 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 3-4 days |
| Harvest ： | cell harvest of ca. 8 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 8% polyploidy - 48(45-49)<2n>XX, +12, +13 - no consistent structural abnormality was detected |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |