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| 产品名称： | PL-21 |
|---|---|
| 商品货号： | TS259812 |
| <strong>Cell line </strong>： | PL-21 |
| DSMZ no. ： | TS259812 |
| Species ： | confirmed as human by cytogenetics and STR typing |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from the peripheral blood of a 24-year-old man with refractory acute promyelocytic leukemia after mediastinal granulocytic sarcoma; however, PL-21 cells do not carry the t(15;17); based on morphology, surface marker expression of CD14/CD68 and expression of the monocyte-specific esterase, PL-21 cells appear to be rather of monocytic origin; differentiation is inducible by phorpbol ester; cells carry an FLT3 internal tandem duplication |
| Reference(s) ： | 14480, 14690, 14692, 14869 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single cells in suspension; cells have a rather monoblastic morphology without the typical APL granules; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1.0 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days; maintain at ca. 1.0-1.5 x 106 cells/ml; minimum density at ca. 0.5 x 106 cells/ml; maximum density at ca. 2 x 106 cells/ml; cultivate cells in a 12- or 24-well-plate after thawing |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | cell harvest of ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 (+), CD14 -, CD15 +, CD19 -, CD33 +, cyCD68 +, HLA-DR -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertetraploid karyotype - 93(83-96)<4n>XXYY, +3, +5, +5, +6, +6, +7, -9, -10, -11, -12, +13, -14, -17, -21, +22, +22, +mar, del(1)(p11), i(5p)x2-3, der(6)t(6;15)(p25;q24), der(13)ins(13;13)q32;q?q?)x3-4 - no cytogenetic evidence of PML-RARA fusion - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |