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| 产品名称： | ME-1 |
|---|---|
| 商品货号： | TS259814 |
| <strong>Cell line </strong>： | ME-1 |
| DSMZ no. ： | TS259814 |
| Species ： | human (Homo sapiens) |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from the peripheral blood of a 40-year-old Japanese man with acute myeloid leukemia (AML) FAB M4eo at second relapse in 1988; cells were described in the literature to carry the inv(16)(p13q22) leading to the fusion gene CBFB-MYH11. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 15038, 15071, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly in suspension; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1.0 x 106 cells/ml in 24-well-plates; maintain at ca. 0.5-1.0 x 106 cells/ml; split saturated culture 1:2 every 3-5 days; minimum density at ca. 0.5 x 106 cells/ml; we recommend to cultivate cells allways in 12- or 24-well-plates |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 3-5 days |
| Harvest ： | maximum cell density of ca. 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD15 (+), CD19 -, CD33 +, CD34 +, HLA-DR +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 9% polyploidy; 48(45-48)<2n>XY, +8, +20, der(2)t(2;11)(q31;q13), der(9)t(9;18)(q12.2;q11), der(16)t(11;16)(q13;q22)inv(16)(p13q22), der(17)t(2;17)(p25;p12); sdl lacking trisomy 20; carries inversion/translocation effecting rearrangement of MYH11 and CBFB; resembles published karyotypes |
| Molec. Genetics ： | expression of fusion gene CBFB-MYH11 confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |