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| 产品名称： | JURL-MK2 |
|---|---|
| 商品货号： | TS259817 |
| <strong>Cell line </strong>： | JURL-MK2 |
| DSMZ no. ： | TS259817 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | chronic myeloid leukemia in blast crisis |
| Origin ： | established from the peripheral blood of a 73-year-old man with chronic myeloid leukemia (CML) in blast crisis in 1993; cells carry the Philadelphia chromosome; JURL-MK1 and JURL-MK2 are sister cell lines derived from the same patient |
| Reference(s) ： | 14459 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round single cells growing in suspension, heterogenous in size, occasional giant cells; image |
| Medium ： | 85% RPMI 1640 + 15% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-1.0 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 3-4 days; reduced viability immediately after thawing (ca. 60-70%), but cells will recover thereafter |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 72 hours |
| Harvest ： | cell harvest of ca. 2-3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 -, CD19 -, CD30 +, CD33 +, HLA-DR +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile, sister cell line of JURL-MK1 |
| Cytogenetics ： | human hypotetraploid karyotype with 3.5% polyploidy - 81(77-82)<4n>XXYY, -2, -7, -12, +13, -14, -14, -16, -18, -19, -22, +1-3mar, add(9)(q1)x2, der(9)del(9)(p21.2)t(9;22)(q34;q11)x2, der(11)t(11;9;22)(p15;q34;q11)x2, add(17)(p13)x1-2, der(22)t(9;22)(q34;q11)x2 - carries two copies of Ph effecting BCR-ABL1 fusion together with additional FISH signals on 11p - does not match published karyotype but iteratively resembles JURL-MK1 (sister cell line) |
| Molec. Genetics ： | expression of fusion gene BCR-ABL1 e14-a2 (b3-a2) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |