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| 产品名称： | JJN-3 |
|---|---|
| 商品货号： | TS259820 |
| <strong>Cell line </strong>： | JJN-3 |
| DSMZ no. ： | TS259820 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | plasma cell leukemia |
| Origin ： | established from the bone marrow of a 57-year-old woman with plasma cell leukemia (IgA1kappa) at diagnosis in 1987; JJN-3 is a subclone of the parental cell line JJN-1; plasma cell leukemia is related to multiple myeloma |
| Reference(s) ： | 14486, 14635 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round to oval cells showing a morphological range from mononuclear to occasionally multinucleated cells growing in suspension; image; image |
| Medium ： | 40% Dulbeccos MEM + 40% Iscoves MDM + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.2-0.4 x 106 cells/ml; split saturated culture 1:3 to 1:6 every 2-3 days |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, cykappa +, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotriploid karyotype with 9% polyploidy - 58-67<3n>XX, +1, -2, +5, +8, +8, -9, -11, -12, -13, -15, -17, +20, add(1)(p22), der(1)t(1;?3)(q41;p26)x2, add(3)(p26), add(5)(p15)x1-2, i(5p), del(6)(q13),del(7)(q32), der(7)t(7;11)(q36;q13), add(8)(p1?)x2, t(12;19)(p13;q13), der(14)add(14)(p11)t(14;16)(q32;q23), der(14)t(14;16)(q32;q23), der(16)t(14;16)(q32;q23)x2, del(22)(q12) - carries two copies of t(14;16) associated with c-MAF activation - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |