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| 产品名称： | NOMO-1 |
|---|---|
| 商品货号： | TS259822 |
| <strong>Cell line </strong>： | NOMO-1 |
| DSMZ no. ： | TS259822 |
| Species ： | confirmed as human by cytogenetics and recently by STR fingerprinting |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from the bone marrow of a 31-year-old woman with acute myeloid leukemia (AML FAB M5a) at 2nd relapse; described in the literature to show strong lysozyme activity in the cytoplasm, phagocytic activities, and cells are responsive to induction of differentiation with TPA; cells carry the t(9;11)(p22;q23) KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) alteration |
| Reference(s) ： | 14595, 15031, 14493, 15144, 16555 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells growing in suspension; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1.0 x 106 cells/ml; after thawing cell growth may be retarded; maintain at ca. 0.5 x 106 cells/ml; split saturated culture 1:2 to 1:4 twice a week |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35 hours |
| Harvest ： | cell harvest of ca. 1.5-2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, HLA-DR +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 8% polyploidy - 46-47<2n>XX, +8, -13, +mar, add(7)(q32), der(9)del(9)(p11p13-21)t(9;11)(p22;q23), t(9;13)(q13;q11), der(11)t(9;11)(p22;q23) - carries reciprocal t(9;11) with rearrangement of KMT2A (MLL) - matches published karyotype |
| Molec. Genetics ： | expression of fusion gene KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |