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| 产品名称： | H-2171 |
|---|---|
| 商品货号： | TS259829 |
| <strong>Cell line </strong>： | H-2171 |
| DSMZ no. ： | TS259829 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | small cell lung carcinoma |
| Origin ： | derived from the pleural effusion of a 50-year-old white male with extensive small cell lung carcinoma after chemotherapy in 1989; corresponds to NCI-H2171 |
| Reference(s) ： | 14849, 15046 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | large round cells growing singly or in clumps in suspension |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | split saturated culture 1:2 to 1:3 every 2-4 days; seed out at ca. 1.0 x 106 cells/ml; maintain at 0.7-2.0 x 106 cells/ml; after recovery the growing is slow |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35-60 hours |
| Harvest ： | maximum density at 3.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 8% polyploidy - 42-44<2n>XY, -4, -5, -8, -10, -11, -13, -15, -17, -19, +20, +4-5mar, add(1)(p36), der(2)ins(2;?)(p2?;?)t(2;2)(q37;p24), add(3)(p11), i(3q), del(6)(q25q26), del(9)(p11), der(12)add(12)(p11)del(12)(q24), add(14)(p11), i(15q), add(18)(p11) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |