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| 产品名称： | F-36P |
|---|---|
| 商品货号： | TS259833 |
| <strong>Cell line </strong>： | F-36P |
| DSMZ no. ： | TS259833 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established at diagnosis from the pleural effusion of a 68-year-old man with acute myeloid leukemia (AML M6) secondary to myelodysplastic syndrome (MDS, subtype refractory anemia with excess of blasts, RAEB) in 1989; cells were described in the literature to be proliferatively responsive to GM-CSF and IL-3. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14431, 14482, 18138, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polymorph cells growing as adherent cells and singly in suspension; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 10 ng/ml GM-CSF |
| Subculture ： | seed out at ca. 0.3-0.5 x 106 cells/ml; maintain at ca. 1.0 x 106 cells/ml; split saturated culture 1:3 to 1:8 every 2-5 days; maximum density at ca. 1.8 x 106 cells/ml. Upon cytokine withdrawal cells do not die but undergo a stationary survival status. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24-36 hours |
| Harvest ： | cell harvest of ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, CD41 -, CD42 -, CD71 +, CD235a +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypodiploid karyotype with 6% polyploidy - 41(40-43)<2n>XY, -3, -13, -16, -19, -21, -22, +2mar, ins(X;13)(p11;q?q?), del(3)(p13p24), add(5)(q11), dup(5)(q11q35), der(6)add(6)(p23)dup(6)(q25q27), der(7)add(7)(p13)add(7)(q12), add(9)(q35), der(9)del(9)(q11q34)inv(9)(p2?4;q34), der(10)t(8;10)(q24;p14)t(10;7)(q25;q12), del(11)(q14q21), der(21)t(10;21)(p14;p12) - sideline with dup(1)(p35p36), dup(2)(q1?q2?), del(3)(p13p24) - matches published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |