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| 产品名称： | SKM-1 |
|---|---|
| 商品货号： | TS259835 |
| <strong>Cell line </strong>： | SKM-1 |
| DSMZ no. ： | TS259835 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from the peripheral blood of a 76-year-old Japanese man with acute monoblastic leukemia (AML M5) in 1989 following myelodysplastic syndromes (MDS) |
| Reference(s) ： | 14796, 14797, 14798, 18138 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly in suspension; image; image |
| Medium ： | 80% RPMI 1640 + 15-20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-4 days; maintain at ca. 0.4-2.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | cell harvest of ca. 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 (+), CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, HLA-DR +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 8% polyploidy - 43(38-43)<2n>XY, +1, -12, -14, -20, -21, t(1;19)(q21;q13), del(2)(p11), del(9)(q12), add(17)(p1?) - sideline with idem, der(10)t(10;?21)(p11;q11), -21 - consistent with published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |