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| 产品名称： | KOPN-8 |
|---|---|
| 商品货号： | TS259844 |
| <strong>Cell line </strong>： | KOPN-8 |
| DSMZ no. ： | TS259844 |
| Species ： | confirmed as human by PCR speciation and cytogenetics |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established from the peripheral blood of a 3-month-old girl with B cell precursor acute lymphoblastic leukemia (BCP-ALL) of type B-III in 1977; cells were described in the literature to carry the t(11;19)(q23;p13) leading to the KMT2A-MLLT1 (MLL-MLLT1; MLL-ENL) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14493, 14744, 15153, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round rather small cells growing in suspension; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1.0 x 106 cells/ml in a 12-well-plate with 20% FBS; maintain at ca. 0.5-1.5 x 106 cells/ml; split saturated culture 1:3 to 1:5 every 3 days; the viability may drop to about 10% after thawing, but the cells will recover thereafter; we recommend to centrifuge the cells in order to remove cellular debris |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | cell harvest of ca. 2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD38 +, CD80 -, CD138 -, HLA-DR +, cyIgG -, cyIgM +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 4% polyploidy - 45(42-45)<2n>XX, -14, t(8;13)(q24;q21.2), t(11;19)(q23;p13), der(13)t(13;14)(p11;q11) - carries t(11;19) effecting KMT2A-MLLT1 (MLL-MLLT1; MLL-ENL) fusion - matches published karyotype |
| Molec. Genetics ： | expression of fusion gene KMT2A-MLLT1 (MLL-MLLT1; MLL-ENL) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |