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| 产品名称： | AML-193 |
|---|---|
| 商品货号： | TS259847 |
| <strong>Cell line </strong>： | AML-193 |
| DSMZ no. ： | TS259847 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from a 13-year-old girl with acute myeloid leukemia (AML FAB M5) at relapse |
| Reference(s) ： | 14703, 14482 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorph cells growing singly or in small clusters in suspension; image; image |
| Medium ： | 70% Iscoves MDM + 10% h.i. FBS + 20% vol conditioned medium of cell line 5637 (DSMZ ACC 35) |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-1.0 x 106 cells/ml; split saturated culture 1:2 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | cell harvest of ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 -, CD15 +, CD19 -, CD33 +, CD34 -, HLA-DR -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 4% polyploidy - 49(46-52)<2n>XX, +3, +6, +8, +13, i(17q) - matches published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |