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| 产品名称： | SC-1 |
|---|---|
| 商品货号： | TS259848 |
| <strong>Cell line </strong>： | SC-1 |
| DSMZ no. ： | TS259848 |
| Species ： | confirmed as human by PCR speciation and cytogenetics |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the ascitic fluid of a 67-year-old man at diagnosis of B cell lymphoma (B-NHL, follicular lymphoma, small cleaved cell type) in 1977 |
| Reference(s) ： | 14951, 15013 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | lymphoid cells growing singly or in small clumps in suspension; image; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 1.5 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 3-5 days; maintain at 1-2 x 106 cells/ml; max. density at ca. 3.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD37 +, CD38 +, cyCD79a (+), CD80 +, CD138 +, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa -, sm/cy lambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 4% polyploidy - 47(43-48)<2n>XY, +7, -17, +mar, add(3)(q27), t(8;14;18)(q24;q32;q21), add(14)(q32) - carries 3-way t(8,14,18) effecting genomic co-amplification of BCL2 and MYC - resembles published karyotype |
| Molec. Genetics ： | expression of fusion gene BCL2-IGH confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |